Pseudovirus-based Neutralization Test (PVNT)
The Pseudovirus-based Neutralization Test (PVNT) has emerged as a robust and versatile alternative to traditional virus neutralization assays in modern virology. By utilizing recombinant viral vectors (such as Lentivirus or VSV) engineered to display the surface glycoproteins of highly pathogenic viruses, PVNT allows for the precise measurement of neutralizing antibodies without the need for the live, infectious agent. This system provides a safer, high-throughput platform that can be conducted in Biosafety Level 2 (BSL-2) facilities, significantly accelerating vaccine and antibody evaluation studies.