Dr. E. Sreekumar

Director

Dr. E. Sreekumar has 20 years’ experience in Medical Virology and his research focuses on Dengue virus and Chikungunya virus biology. His studies elucidated the genetic profile of these viruses circulating in the region.; and during his tenure at RGCB his team has established an animal model for dengue, which is being used for evaluating drugs to treat dengue shock syndrome, dengue vaccine candidates and antiviral molecules. His team also focuses on developing the paradigm of host-directed antiviral therapy focusing on Interferon-stimulated gene modulation; and has established assays for identifying such modulators. At IAV, he has built a core research capacity in the field of monoclonal antibodies and. antiviral development against emerging viruses.

Professional appointments

2021- present: Director

Institute of Advanced Virology (IAV)

2019-2021: Scientist F

Molecular Virology Laboratory, Rajiv Gandhi Centre for Biotechnology

2015-2016: Visiting Faculty

Johns Hopkins Bloomberg School of Public Health, Baltimore, USA Molecular Microbiology and Immunology

2015-2019: Scientist EII

Molecular Virology Laboratory, Rajiv Gandhi Centre for Biotechnology

2011-2015: Scientist EI

Molecular Virology Laboratory, Rajiv Gandhi Centre for Biotechnology

2007-2001: Scientist C

Molecular Virology Laboratory, Rajiv Gandhi Centre for Biotechnology

2004-2007: Scientist B

Molecular Virology Laboratory, Rajiv Gandhi Centre for Biotechnology

1997-1998: Veterinary Surgeon

Animal Husbandry Department, Kerala

Education

2004: PhD Biotechnology

Rajiv Gandhi Centre for Biotechnology (Kerala University)

1996: MVSc Veterinary Immunology

Indian Veterinary Research Institute, Bareilly

1994: BVSc. & A.H

A.H- Veterinary Science, College of Veterinary & Animal Sciences, Mannuthy, Trichur

Scientific discipline

Molecular Virology

Honors & Awards

2015 – 2016

Fulbright-Nehru Academic and Professional Excellence Fellowship, US-India Educational Foundation

2004

ISVIB Young Scientist Award

1994 – 96

IVRI Gold Medal and Best Student Award

Contact

Research Summary

The current research of his laboratory focuses on developing well standardized assaysvfor antiviral screening and immune response evaluation against emerging flaviviruses. The research employs surrogate systems such as pseudovirus-based assays that can be performed in BSL-2 facilities as well classical assays such as plaque reduction assays. His laboratory also focus on developing host-directed antivirals focusing on the modulation of Interferon- stimulated genes. In one of the studies, his team tries to identify the role of Interferon- stimulated transmembrane proteins (IFITMs) in dengue virus infection and how to effectively use their modulation in developing strategies to develop antivirals.

Current Research Grants

2024 – 2029

DBT-SAHAJ Viral Bio-assay Facility (VBaF) for Recombinant Monoclonal Antibody Generation and Pseudovirion-Based Services for Vaccine Response and Therapeutics Evaluation. Department of Biotechnology, Govt of India

2020 – 2023

Design and Synthesis of Novel Iminosugar variants and their cationic amphiphiles as antiviral therapeutics against Dengue virus (DENV). Department of Biotechnology, Govt of India

2022 – 2025

Synthesis of polyhydroxylated pyrrolidine iminocyclitol-based novel antivirals against Dengue Virus. Indian Council of Medical Research (ICMR)

2024 – 2026

Development of a rapid and reliable invitro screening platform based on fluorescent reporter knock in targeting host cell IFITM gene to identify phytochemical immunomodulators against dengue viruses.

Completed research grants

2017 – 2020

Elucidation of the role of endothelial cell signaling pathways in vascular permeability modulation in Dengue virus infection. Indian Council of Medical Research (ICMR)

2017 – 2020

Identification of cellular pathways differentially modulated in Human microvascular endothelial cells upon Dengue virus infection. Kerala State Council for Science, Technology & Environment (KSCSTE)

2015 – 2020

Antivirals from medicinal plants of Western Ghats selected based on traditional knowledge (TK) / Ethnomedical information. Department of Biotechnology, Govt of India

2020 – 2021

New Therapeutics against SARS Cov2: Analyzing small molecule chemical libraries by establishing targeted cell-based assays for inhibitors of viral entry & and viral protease. Department of Biotechnology, Govt of India

2019 – 2022

Therapeutic targeting endothelial kinases to abrogate Dengue virus-induced vascular permeability. Indian Council of Medical Research (ICMR)

Selected Publications

Aneesh B, Pillai SK, Chippy PS, Chandran M, Jose AV, Kailas L, Neziya M, Aswathyraj S, Sreekumar E. Human bocavirus infections in paediatric patients in a tertiary care hospital in Kerala, India. Arch Virol. 2025 Jan 10;170(2):36.

Vijayan A, Sukumaran A, Jones S, Paul A, Ahmed S, Mehta P, Mohanan M, Santhosh Kumar, Sreekumar E, Shenoy P. (2024) Boosting Vaccine Response in Autoimmune Rheumatic Disease Patients With Inadequate Seroconversion: An Analysis of the Immunogenicity of Vector-Based and Inactivated Vaccines. Cureus 16(3): e55764.

Modak A, Mishra SR, Awasthi M, Aravind A, Singh S, Sreekumar E (2024). Fingolimod (FTY720), an FDA-approved sphingosine 1-phosphate (S1P) receptor agonist, restores endothelial hyperpermeability in cellular and animal models of dengue virus serotype 2 infection. IUBMB Life.76(5):267-285. doi: 10.1002/iub.2795.

Indu P S, Anish T S, Chintha S, Libu G K, Tony L, Siju NS, Sreekumar E, Santhoshkumar A, Aravind R, Saradadevi K L, Sunija S, J, Anupriya M G, Mathew T, Reena K J, Meenakshy M, PNamitha P, Pradeep Kumar N, Roop Kumari, Mohamed A J, Nagpal B, SSarkar S, Sadanandan R, Velayudhan R. (2023). The Lancet Regional Health-Southeast Asia. Lancet Reg Health Southeast Asia. 11;22:100337.

Kaviyil JE, Raja K, Gaitonde R, Easwaran S, Kala VK, Ponnambath DK (2023). Targeted Sanger Sequencing of a Cluster of COVID-19 Cases in the Surgical ICU of a Non-COVID Hospital: Lessons Learned. Cureus15(9): e44755. doi:10.7759/cureus.44755

Jancy SV, Lupitha SS, Chandrasekharan A, Varadarajan SN, Nelson-Sathi S, Prasad R, Jones S, Easwaran S, Darvin P, Sivasailam A, Santhoshkumar TR (2023). A high- throughput screening system for SARS-CoV-2 entry inhibition, syncytia formation and cell toxicity. BiolProced Online. 25(1):22. doi: 10.1186/s12575-023-00214-1.

Pradeep P, Sivakumar KC, Sreekumar E (2023). Host Factor Nucleophosmin 1 (NPM1/B23) Exerts Antiviral Effects against Chikungunya Virus by Its Interaction with Viral Nonstructural Protein 3 Microbiol Spectr.11(4): e0537122. doi:10.1128/spectrum.05371-22.

Modak A, Mishra SR, Awasthi M, Sreedevi S, Sobha A, Aravind A, Kuppusamy K, Sreekumar E (2023). Higher-temperature-adapted dengue virus serotype 2 strain exhibits enhanced virulence in AG129 mouse model. FASEB J. 37(8):e23062. doi: 10.1096/fj.202300098R.

Sagna A, Nair RVR, Hulyalkar N, Rajasekharan S, Nair VTG, Sivakumar KC, Suja SR, Baby S, Sreekumar E (2023). Ethyl palmitate, an anti-chikungunya virus principle from Sauropusandrogynus, a medicinal plant used to alleviate fever in ethnomedicine. J.Ethnopharmacol. 309:116366

Chandran D, Sreekumar E, Prajitha KC, Sharahudeen A, Raveendran CL; Research team (2022). Breakthrough infection with SARS-CoV-2 delta variant in old-age homes in a Southern District of Kerala, India. Indian J Public Health. 66(Supplement):S36- S40. doi: 10.4103/ijph.ijph_1084_22.

Lupitha SS, Darvin P, Chandrasekharan A, Varadarajan SN, Divakaran SJ, Easwaran S, Nelson-Sathi S, Umasankar PK, Jones S, Joseph I, Pillai MR, Santhoshkumar TR (2022). A rapid bead-based assay for screening of SARS-CoV-2 neutralizing antibodies. Antib Ther.5(2):100-110. doi: 10.1093/abt/tbac007.

Nair SR, Abraham R, Sreekumar E (2022). Generation of a Live-Attenuated Strain of Chikungunya Virus from an Indian Isolate for Vaccine Development. Vaccines (Basel).10(11):1939.

Rugma R, Valsan C, Sreekumar E, Sathiavathy KA (2022). Molecular detection of dengue virus serotypes prevalent in central Kerala and its correlation with disease severity. Indian J Microbiol Res.9(1):55-61.

Sankaradoss A, Jagtap S, Nazir J, Moula SE, Modak A, Fialho J, Iyer M, Shastri JS, Dias M, Gadepalli R, Aggarwal A, Vedpathak M, Agrawal S, Pandit A, Nisheetha A, Kumar A, Bordoloi M, Shafi M, Shelar B, Balachandra SS, Damodar T, Masika MM, Mwaura P, Anzala O, Muthumani K, Sowdhamini R, Medigeshi GR, Roy R, Pattabiraman C, Krishna S, Sreekumar E*(2022). Immune profile and responses of a novel dengue DNA vaccine encoding an EDIII-NS1 consensus design based on Indo- African sequences. Mol Ther. 30(5):2058-2077. doi: 10.1016/j.ymthe.2022.01.013.

Nelson-Sathi S, Umasankar PK, Sreekumar E, Nair RR, Joseph I, Nori SRC, Philip JS, Prasad R, Navyasree KV, Ramesh S, Pillai H, Ghosh S, Santosh Kumar TR, Pillai MR (2022). Mutational landscape and in silico structure models of SARS-CoV-2 spike receptor binding domain reveal key molecular determinants for virus-host interaction. BMC Mol Cell Biol. 23(1):2. doi: 10.1186/s12860-021-00403-4.

Team

Team Members

Dr. Geetu Rose Varghese

Principal Investigator

geeturose@d1.iav.res.in

Mrs. Sreeja S

Technical Officer

sreeja@d1.iav.res.in

Mrs. Karthika S Nair

Project Associate

sreekarthikasnair@gmail.com

Ms. Aarya J Anil

PhD Student (UGC-JRF)

aaryajanil@gmail.com

Ms. Liby Ann Mathew

Project Associate

libyann2441995@gmail.com

Dr. Nanditha Nair G

Project Scientist 

nanditha@d1.iav.res.in

Ms. Varsha Iyengar

Project Associate I

varshaiyengar02@gmail.com

Ms. Athira

Project Associate

paruathira789@gmail.com

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